abstract：:A new enzymatic activity, which catalyses formation in vitro of di-O-(indole-3-acetyl)-D-glucose from 1-O-(indole-3-acetyl)-beta-D-glucose has been found in extracts of Zea mays seedlings. The structure of di-O-(indole-3-acetyl)-D-glucose, not as yet described, has been assigned by GC-MS, 1H NMR and ammonolysis. ...

journal_title：Acta biochimica Polonica

authors： Szmidt-Jaworska A,Kesy J,Kopcewicz J

更新日期：1997-01-01 00:00:00

abstract：:The nature of genotoxic and cytotoxic effects induced by hydralazine was analyzed taking into account possible protection of cells by catalase, superoxide dismutase and dimethyl sulfoxide. For the experiments designed to evaluate the influence of scavengers on the genotoxicity expressed as the SOS induction factor the...

journal_title：Acta biochimica Polonica

authors： Marczewska J,Koziorowska JH

更新日期：1997-01-01 00:00:00

abstract：:New blocking group combinations for the machine-aided oligoribonucleotide synthesis on solid phase material have been developed and tested regarding their general application. An acetal function for 2'-OH protection offers a series of advantages in the synthetic approach but special conditions have to be fulfilled in ...

journal_title：Acta biochimica Polonica

authors： Pfleiderer W,Matysiak S,Bergmann F,Schnell R

更新日期：1996-01-01 00:00:00

abstract：:The mammalian deoxyribonucleoside kinases thymidine kinase 1 and 2, deoxycytidine kinase and deoxyguanosine kinase phosphorylate deoxyribonucleosides and provide an alternative to de novo synthesis of DNA precursors. Their activities are essential for activation of several chemotherapeutically important nucleoside ana...

journal_title：Acta biochimica Polonica

authors： Johansson NG,Eriksson S

更新日期：1996-01-01 00:00:00

abstract：:Efficient synthesis of two small eukaryotic polypeptides of human and plant origin was carried out using a novel expression/secretion yeast vector, pYET. The yield was optimized in respect of the yeast strain, expression cassette construction, promoter regulation and culture conditions. Both cloned genes code for biot...

journal_title：Acta biochimica Polonica

authors： Topczewska J,Rempoła B,Fikus M

更新日期：1996-01-01 00:00:00

abstract：:A set of metabolite carriers operates the traffic of numerous molecules consumed or produced in mitochondrial matrix and/or cytosolic compartments. As their existence has been predicted by the chemiosmotic theory, the first challenge, in the late sixties, was to prove their presence in the inner mitochondrial membrane...

journal_title：Acta biochimica Polonica

authors： Sluse FE

更新日期：1996-01-01 00:00:00

abstract：:The immunodiffusion cross-reactivity and competitive inhibition ELISA assays were used for immunological differentiation of latent form, cleaved form and guanidinium hydrochloride (GuHCl) induced polymer of human alpha 1-proteinase inhibitor (alpha 1-PI). Under the conditions studied, the differences between latent fo...

journal_title：Acta biochimica Polonica

authors： Wawrzos I,Kitagawa Y,Kołoczek H

更新日期：1996-01-01 00:00:00

abstract：:Plasma membranes of rat platelets produced at normal platelet counts and during early recovery from immune-mediated thrombocytopenia were examined for the contents of carbohydrates, lipids and glycosphingolipids. Glucosylceramide, two monosialo-gangliosides and one disialo-ganglioside were found to be the major glycos...

journal_title：Acta biochimica Polonica

authors： Zdebska E,Soszyńska B,Dobrowolski Z,Kościelak J

更新日期：1996-01-01 00:00:00

abstract：:In developing Galleria mellonella larvae (reared at 30 degrees C) three proteins of 74, 76 and 81/82 kDa were identified. They represent a group of storage proteins (LHP proteins). In Galleria larvae, the development of which is arrested by low temperature (18 degrees C), accumulation of the 74, 76 and 81/82 kDa prote...

journal_title：Acta biochimica Polonica

authors： Kłudkiewicz B,Godlewski J,Grzelak K,Cymborowski B,Lassota Z

更新日期：1996-01-01 00:00:00

abstract：:The early molecular events of glucocorticoid-induced apoptosis have been investigated by studying glucocorticoid receptor levels, as well as binding activities to GRE and AP-1 sequences, using nuclear extracts from dexamethasone (Dex)-treated rat thymocytes. When the time-course of glucocorticoid-receptor complexes in...

journal_title：Acta biochimica Polonica

authors： Sikora E,Rossini GP,Grassilli E,Bellesia E,Salomoni P,Franceschi C

更新日期：1996-01-01 00:00:00

abstract：:Immunoglobulins anti-endonuclease 32 kDa inhibit DNA synthesis. We observed that low concentrations of IgGs (about 50 micrograms IgG per 1 x 10(6) cell nuclei) temporary inhibit DNA synthesis. This inhibition concerns only the synthesis of DNA bound to the nuclear matrix (associated with isolated nuclear matrix). Prei...

journal_title：Acta biochimica Polonica

authors： Rzepecki R,Szopa J

更新日期：1995-01-01 00:00:00

abstract：:Replication of lambda plasmid DNA is halted in amino acid-starved wild type (stringent) strains whereas it proceeds in relA (relaxed) mutants. The only transcription which could be important in lambda plasmid DNA replication in amino acid-starved Escherichia coli cells is that starting from the pR promoter. Using a fu...

journal_title：Acta biochimica Polonica

authors： Szalewska-Pałasz A,Wegrzyn G

更新日期：1995-01-01 00:00:00

abstract：:The walls of human abdominal aortas and atherosclerosis-induced aneurysms contain similar amounts of collagen. The quantitative ratio between collagens of various types of this protein does not differ significantly either, whereas solubility of the collagen in aneurysmal wall and its susceptibility to the action of ED...

journal_title：Acta biochimica Polonica

authors： Sobolewski K,Wolańska M,Bańkowski E,Gacko M,Głowiński S

更新日期：1995-01-01 00:00:00

abstract：:The PCR conditions have been optimized to make the process faster and more economical. When short DNA fragments are to be amplified, the time of denaturation, annealing and extension steps can be as short as 1 s each, and the yield of PCR product is still high, sufficient for many types of analysis. The PCR can be don...

journal_title：Acta biochimica Polonica

authors： Sobczak K,Kozłowski P,Krzyzosiak WJ

更新日期：1995-01-01 00:00:00

abstract：:The integration host factor (IHF) is a sequence-specific, histone-like, multi-functional DNA-binding and -bending protein of Escherichia coli. Characterization and functional analysis of this protein has been carried out mainly in bacteriophage lambda and other mobile genetic elements. In this paper we report data con...

journal_title：Acta biochimica Polonica

authors： Hiszczyńska-Sawicka E,Kur J

更新日期：1995-01-01 00:00:00

abstract：:Isoelectric focusing of homogenous arylsulfatase B from human placenta pointed to the presence of enzymatically active and inactive forms of high pI (pH 9-8) and of lower pI (pH 6.5-5.5). Glycan chain analysis performed with the use of a Glycan Differentiation Kit showed that basic forms of arylsulfatase B from human ...

journal_title：Acta biochimica Polonica

authors： Laidler P,Gałka-Walczak M,Lityńska A

更新日期：1995-01-01 00:00:00

abstract：:The phosphatidylinositol-3-kinases (PtdIns-3-kinase) are a family of enzymes involved in the control of cell replication. One member of the family, the mammalian p110/p85 PtdIns-3-kinase, is a potential target for anticancer drug development because of its role as a component of growth factor and oncogene activated si...

journal_title：Acta biochimica Polonica

authors： Powis G,Berggren M,Gallegos A,Frew T,Hill S,Kozikowski A,Bonjouklian R,Zalkow L,Abraham R,Ashendel C

更新日期：1995-01-01 00:00:00

abstract：:The yeast cell wall as a good antifungal target is discussed in general. More specifically the reaction, catalyzed by Dol-P-Man: protein O-D-mannosyltransferase is proposed as a new potential target. Six genes responsible for this endoplasmic reticulum-localized reaction have been cloned and characterized so far. Trip...

journal_title：Acta biochimica Polonica

authors： Tanner W,Gentzsch M,Immervoll T,Scheinost A,Strahl-Bolsinger S

更新日期：1995-01-01 00:00:00

abstract：:Geranylgeranyl diphosphate (GGPP) synthase was purified to homogeneity from bovine brain in a one-step affinity column procedure. For the construction of the affinity column, a farnesyl diphosphate (FPP) analog, O-(6-amino-1-hexyl)-P-farnesylmethyl phosphonophosphate, was synthesized and linked to the spacer of the ma...

journal_title：Acta biochimica Polonica

authors： Sagami H,Morita Y,Korenaga T,Ogura K

更新日期：1994-01-01 00:00:00

abstract：:Time course of glucose binding by histone Hl and total histones was followed in isolated histone preparations and in thymus nuclei. In both cases the uptake of glucose by Hl was surprisingly high in contrast to a much lower uptake of glucose by total histones. DNA is not implicated in glycation of histones in nuclei. ...

journal_title：Acta biochimica Polonica

authors： Lakatos A,Kiss N,Jobst K

更新日期：1994-01-01 00:00:00

abstract：:In this paper transcriptional factor IIIA (TFIIIA) has been used as a probe for identity of three-dimensional-structure of eukaryotic 5S rRNAs. I was interested in finding a common motif in plant and Xenopus 5S rRNAs for TFIIIA recognition. I found that the two eukaryotic 5S rRNAs (from wheat germ and lupin seeds) are...

journal_title：Acta biochimica Polonica

authors： Barciszewska MZ

更新日期：1994-01-01 00:00:00

abstract：:AMP deaminase (EC 3.5.4.6) from anterior lobe of bovine pituitary has been purified for the first time. Six molecular forms of the enzyme were eluted from phosphocellulose P11 with a KC1 concentration gradient. By two stage gel chromatography individual molecular forms were purified to electrophoretic homogeneity. Com...

journal_title：Acta biochimica Polonica

authors： Sharoyan S,Mardanian S,Haroutunian A

更新日期：1994-01-01 00:00:00

abstract：:The activity of the pyruvate dehydrogenase complex (PDC) purified from pig kidney medulla was affected by K+, Na+, Cl-, HCO3-, HPO4(2-) and changes in ionic strength. Increased ionic strength influenced the activity of PDC from medulla by decreasing the Vmax and S0.5 for pyruvate and increasing the Hill coefficient. T...

journal_title：Acta biochimica Polonica

authors： Pawełczyk T,Olson MS

更新日期：1993-01-01 00:00:00

abstract：:The comparative studies were performed on insulin receptors of erythrocytes and liver plasma membranes in fish (tench and carp) and bird (duck). The Scatchard plots indicated the presence of two pools of binding sites both in fish and duck. These pools show inter-species differences in binding ability and the number o...

journal_title：Acta biochimica Polonica

authors： Nowak KW,Maćkowiak P,Nogowski L

更新日期：1993-01-01 00:00:00

abstract：:In vitro addition of 16,16'-dimethyl prostaglandin E2 to Golgi-rich membrane fraction in final concentration of 0.1 microgram/1 mg of protein increased generally the activity of galactosyltransferase in comparison with control. The percentage of phospholipids in the whole fraction was similar in both investigated grou...

journal_title：Acta biochimica Polonica

authors： Kordowiak AM,Tomecki J,Procyk K,Kapusta P

更新日期：1993-01-01 00:00:00

abstract：:Genotoxic activities of flavonoids (quercetin, rhamnetin, isorhamnetin, apigenin, luteolin) were investigated using two short-term bacterial assays. In the "repair test" in Salmonella typhimurium (strains TA1538 uvrB- and TA1978 uvrB+) the flavonoids studied did not introduce any damage into the DNA recognized by UvrA...

journal_title：Acta biochimica Polonica

authors： Czeczot H,Kusztelak J

更新日期：1993-01-01 00:00:00

abstract：:The erythrocytes from Morris Hepatoma 5123 bearing rats took up Na+ and K+ ions from the incubation medium and released Na+ into the extracellular space at lower rates than did erythrocytes from intact control rats. The lipid composition of erythrocytes membranes from the tumor-bearing rats differed from that of membr...

journal_title：Acta biochimica Polonica

authors： Batko J,Płotast-Necas B,Warchoł T,Karoń H

更新日期：1992-01-01 00:00:00

abstract：:The activities of six enzymes associated with carbohydrate metabolism were measured both in carcinomas and in normal breast tissues. The following differences were observed. 1. The carcinoma showed higher enzyme activities than the normal mammary tissue. 2. The ratios of glutamate dehydrogenase, hydroxybutyrate dehydr...

journal_title：Acta biochimica Polonica

authors： Rzymowska J

更新日期：1992-01-01 00:00:00

abstract：:Initiation of replication of lambda DNA requires assembly of the proper nucleoprotein complex consisting of the lambda origin of replication-lambda O-lambda P-dnaB proteins. The dnaJ, dnaK and grpE heat shock proteins destabilize the lambda P-dnaB interaction in this complex permitting dnaB helicase to unwind lambda D...

journal_title：Acta biochimica Polonica

authors： Osipiuk J,Zylicz M

更新日期：1991-01-01 00:00:00

abstract：:We found that it is possible to use eriochrome black T as a dye for agarose gel electrophoresis of DNA. The presence of the dye does not change the migration rate of DNA and does not influence the electrophoretical picture. ...

journal_title：Acta biochimica Polonica

authors： Obuchowski M,Wegrzyn G

更新日期：1991-01-01 00:00:00

abstract：:The SOS-Chromotest was used to detect DNA damage induced by two flavones: apigenin and luteolin and/or their metabolites. It was found that the compounds tested weakly induced the SOS repair system in the strain studied. ...

journal_title：Acta biochimica Polonica

authors： Czeczot H,Bilbin M

更新日期：1991-01-01 00:00:00

abstract：:Basing on the use of highly specific antibodies it has been proved that ACTH induces synthesis of cytochrome P-450 and adrenodoxin in cultured adrenal cortex cells. ...

journal_title：Acta biochimica Polonica

authors： Bogusz E,Trzeciak WH

更新日期：1991-01-01 00:00:00

abstract：:Murine leukemia L1210 cells contain active glucosamine 6-phosphate synthase inhibited by N3-4-methoxyfumaroyl-L-2,3-diaminopropanoic acid (FMDP). However, FMDP-peptides do not exhibit any cytotoxicity against these cells, HeLa S3 human cervical carcinoma cells, and LL2 murine Lewis lung carcinoma cells. It is suggeste...

journal_title：Acta biochimica Polonica

authors： Bontemps-Gracz M,Milewski S,Borowski E

更新日期：1991-01-01 00:00:00

abstract：:In the haemolymph of Oniscidea haemocyanin exists mainly in the form of hexamer 16S (90%); the 5S and 24S components are present in small amounts. Alkaline dissociation of the examined 16S haemocyanins is dependent on pH and divalent cations (Ca2+,Mg2+). The 16S component consists of 23-24.5% acidic amino acids, 15-18...

journal_title：Acta biochimica Polonica

authors： Helszer Z,Gondko R

更新日期：1991-01-01 00:00:00

abstract：:A series of 9-aminoacridine carboxamide derivatives of systematically varied structure was assayed in an RNA synthesis in vitro system. Escherichia coli DNA-dependent RNA polymerase and DNA derived from phage T7 or calf thymus were used to measure the effect of the drugs on overall RNA and the initiating dinucleotide ...

journal_title：Acta biochimica Polonica

authors： Piestrzeniewicz MK,Czyz M,Denny WA,Gniazdowski M

更新日期：1990-01-01 00:00:00

abstract：:Monoclonal antibodies (MAb 1-7-1) directed against the isoenzymes of rat liver cytochrome P-450 induced by methylcholanthrene, inhibited benzo(a)pyrene hydroxylase less strongly at low (0.04 mM) than at high (2 mM) NADH concentration. Inhibition of NADPH dependent hydroxylation was the same irrespective of NADPH conce...

journal_title：Acta biochimica Polonica

authors： Brauze D,Mikstacka R,Pelkonen O

更新日期：1990-01-01 00:00:00

abstract：:A method has been developed for functional reconstitution of the isolated pyruvate carrier. Optimal conditions were reached after removal of deoxycholate with 27 mg Triton X-100 per gram of moist Amberlite XAD-2 beads and 7 micrograms protein/mg of phospholipid. Uptake of pyruvate into proteoliposomes and sensitivity ...

journal_title：Acta biochimica Polonica

authors： Nałecz KA,Zambrowicz BE

更新日期：1990-01-01 00:00:00

abstract：:The effect of vitamin D3 on the activity of different forms of alkaline phosphatase as well as on the ability of their subunits to form phosphorylated intermediates in the presence of [gamma-32P]ATP was studied. In experiments with rats fed a diet enriched in vitamin D3 phosphatase F3 activity was doubled and the radi...

journal_title：Acta biochimica Polonica

authors： Dupuis Y,Tardivel S,Ranivosoa A,Fournier P,Razanamaniraka L,Zelazowska E,Porembska Z

更新日期：1990-01-01 00:00:00

abstract：:Trans-diamminedichloroplatinum(II) (trans-DDP) forms with DNA at 37 degrees C, more numerous interstrand cross-links than cis-DDP in the isolated DNA and DNA in the chromatin complex. An increase in the temperature to 42.5 degrees C had no effect on the interstrand cross-links of DND-Pt-DNA formed by the two isomers, ...

journal_title：Acta biochimica Polonica

authors： Kulamowicz I,Walter Z,Krupa R

更新日期：1990-01-01 00:00:00

abstract：:Mitochondria were prepared from rat liver, solubilized with Triton X-100 and submitted to chromatography on dry hydroxylapatite. The pass-through from the column, containing a mixture of mitochondrial carriers, was processed further by triazine dye affinity chromatography and optimal conditions for binding and elution...

journal_title：Acta biochimica Polonica

authors： Szewczyk A,Poddana H,Nałecz MJ

更新日期：1990-01-01 00:00:00